Fluorescent Quantitative PCR Instrument

The quantitative PCR instrument is primarily used to perform real-time quantitative PCR experiments. By stimulating and collecting fluorescence signals, it enables real-time monitoring of the experimental process. Experimental data is plotted into fluorescence curves and displayed in real time on the instrument interface. The instrument then performs data fitting and analysis, ultimately generating a PDF-format experimental report.

It enables rapid and precise qualitative and quantitative analysis of target nucleic acids present in biological samples (such as blood or bodily fluids) extracted from other animals. Additionally, it supports analyses of special samples including standard curve fitting, melting curve analysis, and genotyping.

Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA segments. It functions as an in vitro DNA amplification method, comprising a temperature cycle consisting of high-temperature denaturation, low-temperature annealing, and optimal-temperature extension. This process enables rapid amplification of target genes with high specificity, sensitivity, ease of operation, and time efficiency, representing a major innovation in gene amplification technology. PCR can amplify minute amounts of target DNA millions of times, significantly enhancing the analysis and detection capabilities of DNA molecules.

Real-time fluorescent quantitative PCR technology incorporates fluorescent dyes or fluorescently labeled probes into conventional PCR. By monitoring changes in fluorescent signals during the PCR reaction, it enables real-time detection of the entire PCR process. This method uses fluorescent chemicals to track the total product volume after each PCR cycle, allowing quantitative analysis of specific DNA sequences in samples through internal or external controls.

Applications include: fundamental scientific research, pathogen detection, animal disease surveillance, meat adulteration detection, genetically modified organism (GMO) testing, food hygiene inspection, drug development and rational drug use, gene expression analysis, and water quality monitoring.

• Real-time dynamic monitoring: Visualize the entire experimental process with automatically generated fluorescence amplification curves.

• Multi-dimensional analysis: Supports professional analyses including standard curves, melting curves, and genotyping to meet complex detection requirements.

• Features high specificity, sensitivity, ease of operation, and time efficiency.

• High-definition touchscreen operation requires no training for effortless use.

• Efficient report generation with automatic PDF report creation.