PCR Amplifier

The PCR amplifier is primarily used to run real-time fluorescent quantitative PCR experiments. By stimulating and collecting fluorescence, it monitors the experimental process in real time, plots the process data into fluorescence curves displayed on the instrument interface, performs data fitting and analysis, and ultimately generates an experimental report in PDF format.

It enables rapid and precise qualitative and quantitative analysis of target nucleic acids present in biological samples (such as blood and bodily fluids) obtained from other animals. Additionally, it supports analyses of special samples including standard curve fitting, melting curve analysis, and genotyping.

Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA fragments. It functions as an in vitro DNA amplification method, comprising a temperature cycle consisting of high-temperature denaturation, low-temperature annealing, and optimal-temperature extension. This process enables rapid amplification of target genes with high specificity, sensitivity, ease of operation, and time efficiency, representing a major innovation in gene amplification technology.

PCR technology can amplify minute amounts of target DNA millions of times, significantly enhancing the analysis and detection capabilities of DNA molecules.

Real-time fluorescent quantitative PCR technology incorporates fluorescent dyes or fluorescently labeled probes into conventional PCR. By monitoring changes in fluorescent signals during the PCR reaction, it enables real-time detection of the entire PCR process. This method uses fluorescent chemicals to track the total product volume after each PCR cycle, allowing quantitative analysis of specific DNA sequences in samples through internal or external controls.

The instrument finds applications in the following fields: fundamental scientific research, pathogen detection, animal disease surveillance, meat adulteration detection, genetically modified organism (GMO) testing, food hygiene inspection, drug development and rational drug use, gene expression analysis, and water quality monitoring.

• Compact size and lightweight design for easy portability. Effortlessly meets the demands of field experiments.

• Built-in 7-inch HD capacitive touchscreen PDA for intuitive, touch-based operation.

• Android operating system with a clean, intuitive software interface—easy for beginners to master. Pre-set programs enable one-touch experiment initiation.

• High-quality Marlow Peltier cooling elements combined with premium German PT1000 temperature sensors and electrical resistance heating compensation for edge temperature control. Maximum heating rate of 6°C/s and maximum cooling rate of 4°C/s significantly reduce experiment duration.

• Features an efficient heat dissipation system that leverages thermal conductivity to accelerate heat removal during semiconductor cooling, enhancing temperature control speed.

• Electrically resistive heating compensates for edge temperature variations, ensuring superior temperature uniformity.

• Powerful software features include: Fluorescence-based PCR system software; real-time amplification curve monitoring; specimen-specific real-time reaction curve display; real-time temperature curves; data analysis functions; automated positive/negative result determination; graphical display capabilities, and more.