Fluorescent Quantitative PCR Analyzer

The Fluorescent Quantitative PCR Analyzer is primarily used to run real-time fluorescent quantitative PCR experiments. By stimulating and collecting fluorescence, it monitors the experimental process in real time, plots the process data into fluorescence curves displayed on the instrument interface, performs data fitting and analysis, and ultimately generates experimental reports in PDF format.

It enables rapid and precise qualitative and quantitative analysis of target nucleic acids present in biological samples (such as blood or bodily fluids) extracted from other animals. Additionally, it supports analyses of special samples including standard curve fitting, melting curve analysis, and genotyping.

Principle of the Fluorescent Quantitative PCR Detector: Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be viewed as an in vitro method for DNA amplification, consisting of several reaction steps—high-temperature denaturation, low-temperature annealing, and temperature-appropriate extension—forming a thermal cycling cycle. This enables rapid amplification of target genes with high specificity, sensitivity, ease of operation, and time efficiency, representing a major innovation in gene amplification technology.

PCR technology can amplify minute amounts of target DNA millions of times, significantly enhancing the analysis and detection capabilities of DNA molecules.

Real-time quantitative PCR (qPCR) technology incorporates fluorescent dyes or labeled probes into conventional PCR. By monitoring fluorescence signal changes during the reaction, it enables real-time detection of the entire PCR process. This method uses fluorescent chemicals to track the total product volume after each PCR cycle, allowing quantitative analysis of specific DNA sequences in samples through internal or external controls.

Applications include: fundamental scientific research, pathogen detection, animal disease surveillance, meat adulteration detection, genetically modified organism (GMO) testing, food hygiene inspection, drug development and rational drug use, gene expression analysis, and water quality monitoring.

• Features an advanced thermoelectric cooling system for enhanced heating and cooling efficiency.

• Independent fluorescence channels minimize crosstalk between different channels.

• A unique constant-current power supply and 6-zone independent temperature control ensure stable system performance. The optimized light source and optical path design deliver more authentic and reliable fluorescence.

• Comprehensive analysis capabilities paired with customizable reporting formats meet diverse client requirements. The intuitive operating software is easy to learn and use.