African Swine Fever Detection Equipment

The African Swine Fever detection equipment is primarily used to run real-time fluorescent quantitative PCR experiments. Through fluorescence excitation and detection, it monitors the experimental process in real time, plots the experimental data into fluorescence curves displayed on the instrument interface, performs data fitting and analysis, and ultimately generates an experimental report in PDF format.

It enables rapid and precise qualitative and quantitative analysis of target nucleic acids present in biological samples (such as blood or bodily fluids) obtained from other animals. Additionally, it supports analyses of special samples including standard curve fitting, melting curve analysis, and genotyping.

Principle of Classical Swine Fever Detection Equipment: Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA segments. It functions as an in vitro DNA amplification method, comprising a temperature cycle consisting of high-temperature denaturation, low-temperature annealing, and optimal-temperature extension. This process enables rapid amplification of target genes with high specificity, sensitivity, ease of operation, and time efficiency, representing a major innovation in gene amplification technology. PCR can amplify minute amounts of target DNA millions of times, significantly enhancing the analysis and detection capabilities of DNA molecules.

Real-time quantitative PCR technology builds upon conventional PCR by incorporating fluorescent dyes or fluorescently labeled probes. Throughout the PCR reaction, changes in fluorescent signals enable real-time monitoring of the entire process. This method employs fluorescent chemicals to track the total product yield after each PCR cycle, allowing quantitative analysis of specific DNA sequences within the sample through internal or external controls.

Applications include: fundamental scientific research, pathogen detection, animal disease surveillance, meat adulteration detection, genetically modified organism (GMO) testing, food hygiene inspection, drug development and rational drug use, gene expression analysis, and water quality monitoring.

• Compact size, lightweight, and portable, compatible with 0.2PCR single tubes and 8-well strips.

• 13S four-channel rapid light acquisition ensures stable fluorescence signal collection and minimizes delay errors.

• Features a custom Marlow Peltier module-grade semiconductor chip from the USA, employing next-generation semiconductor heating/cooling technology. Achieves a variable temperature rate of up to 7°C per second with a lifespan of one million cycles.

• Powerful software analysis capabilities enable quantitative analysis, melting curve analysis, genotyping, and more. Analysis software includes lifetime free upgrades and supports customized software solutions for various industries.

• Employs proprietary light detection and processing technology to automatically adjust fluorescence background, enhancing fluorescence signal sensitivity and signal-to-noise ratio for superior results.

• Features a maintenance-free, long-life LED light source requiring no replacement. Independent fluorescence channels minimize crosstalk between channels.

• Constant-current control circuit ensures smooth power output, extends Peltier element lifespan, and improves temperature control precision.

• Integrated 10-inch color touchscreen control with overcurrent and overtemperature protection functions.